Journal: Science signaling

Article Title: Extracellular signal–regulated kinase 5 promotes acute cellular and systemic inflammation

doi: 10.1126/scisignal.aaa3206

Figure Lengend Snippet: (A to P) Wild-type mice were treated intraperitoneally with XMD8-92 (50 mg/kg), XMD17-109 (50 mg/kg), BIX02189 (25 mg/kg), or vehicle [30% (2-hydroxypropyl)-β-cyclodextrin with or without 5% DMSO] 30 min before they were injected intravenously with Pam3Cys (2.5 mg/kg), LPS (10 mg/kg), or vehicle (0.9% saline). The plasma concentrations of IL-6, CCL2, and CCL3 and the activity of PAI-1 were quantified 2 hours after challenge. *P < 0.05 when comparing Pam3Cys- or LPS-treated mice in the presence or absence of inhibitor; #P < 0.05 when comparing untreated control mice with mice treated with Pam3Cys or LPS in the presence or absence of inhibitor. Data are means ± SD of four mice per group and are representative of two independent experiments.

Article Snippet: Active PAI-1 was detected with the PAI-1 functional assay ELISA kit (Molecular Innovations).

Techniques: Injection, Activity Assay

Journal: Science signaling

Article Title: Extracellular signal–regulated kinase 5 promotes acute cellular and systemic inflammation

doi: 10.1126/scisignal.aaa3206

Figure Lengend Snippet: (A to D) Wild-type mice were treated intraperitoneally with XMD8-92 (50 mg/kg) or vehicle [30% (2-hydroxypropyl)-β-cyclodextrin] 30 min before being injected intravenously with HKSA (2.5 × 1010 bacteria/kg) or vehicle (0.9% saline). The plasma concentrations of IL-6, CCL2, and CCL3 and the activity of PAI-1 were quantified 2 hours after the mice were challenged. *P < 0.05 when comparing HKSA-treated mice in the presence or absence of inhibitor; #P < 0.05 when comparing untreated control mice with mice treated with HKSA in the presence or absence of inhibitor. Data are means ± SD of four mice per group and are representative of two independent experiments.

Article Snippet: Active PAI-1 was detected with the PAI-1 functional assay ELISA kit (Molecular Innovations).

Techniques: Injection, Activity Assay